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International Journal of Biomedical Science
4(2) 82-88
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© 2005 Master Publishing Group
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Review Article
[FullText]
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Overcoming Hurdles in Hepatitis C Virus Research: Efficient Production of Infectious Virus in Cell Culture |
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Erica Silberstein, Deborah R. Taylor
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Laboratory of Hepatitis and Related Emerging Agents, Center for Biologics Evaluation and Research, US Food and Drug Administration
Corresponding authorDeborah R. Taylor, 8800 Rockville Pike, HFM310, Bethesda, MD 20892. Tel: (301) 827-3660; Fax: (301) 480-7928; E-mail: Deborah.Taylor@FDA.HHS.gov.
Running title: HCV cell culture systems |
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hepatitis C virus; cell culture systems; replicons; particle assembly; viral spread and replication |
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Hepatitis C virus is a flavivirus that infects nearly 2% of the world population. There is no vaccine available and current therapy with interferon and ribavirin is expensive, not well tolerated and effective in only 60% of patients. HCV research has been hampered by the lack of a robust tissue culture system, but recent advances have made virus growth in culture possible. Here we review the current state-of-the-art and the molecular hurdles that have been met and those that still need to be overcome.
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