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International Journal of Biomedical Science
1(1) 53-56
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© 2005 Master Publishing Group
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Original Article
[FullText]
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Effect of bizhongxiao decotion (BZXD) on some cytokines in plasma of rats with CⅡ-induced rheumatoid arthritis |
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WANG An-yu, LIANG Qing-hua, Luo-xu,
BAO Tai-cheng,
LI Chun-yan, CHENG Jiang, LIU Xiao-chun |
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Xiangya Hospital, Central South
University，Changsha 410008 ，China
[1]fund item :the experience was supported by Chinese medicine
administration bureau in Hunan province (20053)and also by science committee in
Hunanprovince(02 ssy4028s)
Correspondence author: LIANG Qing-hua Tel:86-731-4327213
Fax:86-731-4327227 Email:LQHxy@163.com |
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Objective: To investigate the
influence of bizhongxiao decoction(BZXD) which is a Traditional Chinese medicine
for RA including, on the plasma TNF-α and IL-1β in rats with CⅡ-induced
arthritis (CIA) and explore the protective mechanism of BZXD in the treatment of
rheumatoid arthritis .
Methods: 75 SD rats were divided into four groups randomly. Normal
control group (n=5) not be treated any more. The CIA rat was established by
subcutaneous injection with bovine Ⅱ collagen(BⅡC) and complete Freund,s
adjuvant(CFA) after 7d breeding. The CIA rats were divided into the CIA
group(n=16)、BZXD group (n=29) treated with BZXD and the MTX group(n=25) treated
with methotrexate. All rats were killed after various intervals(25,30,35,40,or
45d). At the end of each time interval, we collected the blood of each rat. To
detect TNF-αand IL-1βin plasma with radio-immunity kit. Results: BⅡC and CFA can
be used to copying CIA model. The incidence of arthritis was 88%. The plasma
TNF-αand IL-1βlevels of CIA group, BZXD group and MTX group were notably higher
than those of normal control group(p<0.05), moreover, the CIA group was higher
than those of the MTX group and BZXD group at various interval (p<0.01).
TNF-αand IL-1βrose step by step in CIA group but decreased in BZXD group and MTX
group gradually. Moreover, in BZXD group were lower than those in MTX group
(p<0.05). Conclusion: TNF-αand IL-1βplay a very important role in the formation
and development of RA. BZXD can notably decrease the plasma TNF-αand
IL-1βlevels, which was better than MTX.
Key words: bizhongxiao decotion, methotrexate, arthritis, rheumatoid, TNF-α,
IL-1β
Rheumatoid arthritis (RA) is particular with chronic and symmetric arthritis
which get along with joint damage and matrix erosion. But its mechanism has not
been clear. The recent research has demonstrated that TNF - α and IＬ-1β play key
role in the disease of RA and made progress with matrix erosion. To explore the
mechanism of the RA and the function of BZXD ,we make the collagen-induced
arthritis rat and treated with BZXD, then detect the level of TNF-αand IL-1βin
plasma at various interval
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