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International Journal of Biomedical Science
1(1) 46-52
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© 2005 Master Publishing Group
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Original Article
[FullText]
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Influence of pro- and anti-inflammatory cytokines in Th1 polarization after allogeneic stimulation |
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Silva R.*, Morgado J.M.*,
Freitas A., Couceiro A., Orfão A.,Regateiro
F.,
Paiva A. |
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*These authors contributed equally to this work
Histocompatibility Centre of Coimbra
Short Title: Cytokines effects in Th1 polarization
Corresponding author: Artur Augusto Paiva , Histocompatibility
Centre of Coimbra , Edifício São Jerónimo, 4º Piso, Praceta Mota Pinto
, 3030 Coimbra, Portugal
Fax: 00351239480790 Telef: 00351480700 apaiva@histocentro.min-saude.pt |
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The exogenous cytokine milieu can influence Th1/Th2 polarization. Besides the
differential functional properties, T lymphocytes also acquire distinct profiles
of chemokine receptors. Human Th1 lymphocytes preferentially express CCR5 and
CXCR3 while Th2 lymphocytes express CCR3, CCR4 and CCR8. After their
polarization into Th1 cells, grafted T lymphocytes mediate the development of
graft-vs-host-disease, the major complication after bone marrow transplantation.
We performed mixed lymphocyte cultures for ten days, with and without addition
of IL-2, IL-4, IL-10, IL-12 and IL-18 at the third and sixth day of cultures.
The expression of CXCR3 and CCR5, in CD4+ and CD8+ T lymphocytes was evaluated
by flow cytometry, before and after ten days of culture. The exogenous addition
of IL-2 or IL-12 favoured the Th1/Tc1 phenotype and IL-4 was also capable of
inducing Th1 polarization. In opposition to IL-12, IL-18 didn't induce a
significant polarization into Th1 phenotype, an effect more similar to that
induced by IL-10. This action could explain, at least in part, its possible
protective effect in the incidence of acute and chronic graft-versus-host
disease after allogeneic stem cell transplantation.
Key Words: Flow cytometry; CCR5; Th1/Th2 polarization; GVHD; IL-18
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